Phis2 酵母单杂
Webb1、文库质量评估：理论+涂板评价 交付材料：反馈质量评估结果 2、载体构建：基因合成诱饵基因 构建Phis2载体 交付材料：含诱饵基因的质粒 3、启动子背景筛查：Binding seq基因启动子的背景筛查 交付材料：阳性克隆测序结果 word项目报告 4、文库筛选：诱饵质粒转化和文库质粒转化 酵母筛选和3AT条件优化 筛选结果测序 交付材料：阳性克隆测序结果 … http://www.biofeng.com/zaiti/jiaomuzajiao/pHis2.html
Phis2 酵母单杂
Did you know?
Webb本文针对酵母单杂技术的基本原理、基础知识、主要用途做了简要介绍；并以Y1Hgold（pAbAi-bait）和Y187（pHis2-bait）为例，介绍了自激活检测的目的以及主要 … Webb22 juli 2013 · 酵母单杂交系统 Clontech pHis2说明书 Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker One-Hybrid Library Construction & Screening Kit （#K1617-1）.
Webb1 mars 2024 · VvACTIN or AtACTIN2 was used as internal references for grape and Arabidopsis. The qRT-PCR primers are listed in Table 1. Table 1. Gene-specific primers used for quantitative real-time PCR assays. 2.7. Transformation of Arabidopsis The 35S: VvWRKY30 vector was created by cloning genes into the pSuper1300 binary vector with … Webb2 aug. 2024 · 酵母单杂交技术是在酵母双杂基础上发展而来的一种研究核酸-蛋白相互作用的工具，被广泛用于研究真核细胞内基因的表达调控，如鉴别DNA结合位点发现潜在的 …
Webb19 nov. 2012 · 是选取atg上游2000的片段，然后整个连到phis2载体上吗？ 我之前做了直接取ATG上游1.5k构建到phis2上，感觉不合适。 最好还是找到自己转录因子的结合motif然后取motif和其上下游几十bp的片段，然后串联3个片段构建载体比较合适。 http://www.zoonbio.com/molecular/yeast-one-hybrid-technology.html
Webb9 juli 2024 · 幼儿/小学教育 -- 教育管理. 文档标签：. phis2酵母表达载体说明. 系统标签：. 酵母表达载体 多克隆位点 载体 克隆方法 说明 拷贝. pHis2编号载体名称北京华越洋生物VECT2110pHis2pHis2载体基本信息出品公司:Clontech载体名称:pHis2质粒类型:酵母单杂交载体高拷贝/低拷贝 ...
Webb1 juni 2024 · Clone the products of the PCR reactions carried out in step 3 into the pHIS2 T-vector obtained in step 5. The T-A ligation system includes 0.2 μg of T-vector of pHIS2, 0.5 μL of PCR products (Y1 + Y3 and Y2 + Y3), 1 μL of 10× ligation buffer, 3 U of T4 ligase, and PEG6000 at final concentration of 10% (w/v). howbert surveying ft jennings ohiohttp://www.zoonbio.com/molecular/yeast-one-hybrid.html how many more days till march 4 2023http://www.bioon.com.cn/reagent/show_product.asp?id=3900880 how many more days till march 15thhttp://muchong.com/t-9775583-1 how many more days till march 1WebbY187-pHis2YeastOne-HybridMediaKit（酵母单杂培养基套装YM1010），含有用于Y187-pHis2系统 酵母单杂交文库筛选的全套培养基，性价比更高。培养基以非常方便使用的 … how many more days till june 8thWebbPhis2osgpx1 Promoter Phis2 1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Home > Search Results > TaKaRa > phis2 1. phis2 1 vector TaKaRa is a verified supplier TaKaRa ... howbery farm crowmarshWebb8 juli 2024 · pHis2 编号 载体名称 北京华越洋生物VECT2110 pHis2 pHis2 载体基本信息 出品公司: Clontech 载体名称: pHis2 质粒类型: 酵母单杂交载体 高拷贝/低拷贝: -- 启动子: -- 克隆方法: 多克隆位点，限制性内切酶 载体大小: 7207bp 5' 测序引物及序列: -- 3' 测序引物及序列: -- 载体标签 ... howbery ditch