High-speed counter-current chromatography

WebCountercurrent chromatography (CCC) belongs to the family of liquid partition chromatography but with one distinct feature: the system totally eliminates the use of a … WebJun 28, 2024 · chromatography, high-speed counter-current chromatography (HSCCC) is a continuous and efficient liquid–liquid extractive chro matography with no solid support [27–2 9],

High-speed counter-current chromatography assisted preparative …

WebHigh-speed counter-current chromatography yielded, from 108 mg of the partially purified extract, 53 mg rutin and 32 mg nicotiflorin with 92.5% and 92.2% recovery, with each at over 96.5% purity by high-performance liquid chromatography analysis. Their structures were identified by 1H NMR and 13C NMR. MeSH terms Chromatography, High Pressure Liquid WebDec 1, 2006 · The target compounds isolated and purified by HSCCC were analyzed by high-performance liquid chromatography (HPLC). The separation produced total of 69.2mg of daphnoretin at 99.2% purity, 63.4 mg of 7-methoxy-daphnoretin at 98.7% purity and 58.3 mg of 1,5-diphenyl-1-pentanone at 98.1% purity from 300 mg of the crude extract in one-step … onvoy ghost box https://instrumentalsafety.com

Purification of rutin and nicotiflorin from the flowers of ... - PubMed

WebAug 27, 2024 · All results demonstrated that the high-polarity solvent system with addition of inorganic salt is an effective method for separation of highly polar compounds from natural products by HSCCC. KEYWORDS: High-speed counter-current chromatography highly polar compounds inorganic salt iridoid glycosides Lonicerae japonicae Flos … WebIn this study, eight phenolic compounds were obtained from the over-ground parts of the Aconitum pendulum ( A. pendulum) Busch through an efficient high-speed counter-current chromatography (HSCCC)method. First, the column chromatography for macroporous resin was used for the treatment of crude extract of over-ground parts of A. pendulum … WebOct 3, 2024 · High speed counter current chromatography (HSCCC) utilizes a liquid stationary-phase and, hence, does not suffer from irretrievable adsorption associated … onvoy llc - al

High-Speed Counter-Current Chromatography (HSCCC) …

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High-speed counter-current chromatography

High Performance Countercurrent Chromatography (HPCCC) …

WebJul 26, 2012 · A simple and rapid purification method of 8-methoxy-psoralen from the crude methanol extract of the rhizomes of Pulsatilla chinenis was established using high-speed counter-current chromatography (HSCCC). From the crude extract (233.2 mg), 8-methoxypsoralen (10.0 mg) was separated for the first time in one-step, using a two … WebDec 10, 2024 · HUFA were purified by high-speed counter-current chromatography (HSCCC), characterized by LC-MS and MS/MS, and their antifungal activities were …

High-speed counter-current chromatography

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WebOct 7, 2024 · The high-speed counter-current chromatography (HSCCC) was implemented by employing a two-phase solvent system which consist of n-hexane–ethyl acetate–methanol-water (4:5:4:5, v/v/v/v) and n-hexane–ethyl acetate–methanol-water (1:5:1:5, v/v/v/v) to separate flavonoid into five fractions. WebAug 23, 2024 · This technique was named as High-Speed Countercurrent Chromatography or HSCCC, as the experimental duration was only 3 to 4 hours. Based on the principle of DCCC, Sanki (an instrument engineering ...

WebApr 18, 2008 · High-speed counter-current chromatography (HSCCC) is a liquid–liquid chromatographic technique, which is gaining increasing interest recently. It relies on the use of centrifugal force for the retention of the liquid stationary phase, which is continuously eluted by the counter-flowing mobile phase driven by external pumping [ 7 ]. WebJan 7, 2024 · High-speed counter-current chromatography (HSCCC) was first developed in the early 1980s . It is a kind of chromatographic technology based on the theory of liquid-liquid distribution, and has been widely used in the isolation of natural products for its advantages of high recovery, simple sample preparation, high efficiency as well as ideal ...

WebJul 19, 2024 · High-speed counter current chromatography (HSCCC) is a unique support-free liquid-liquid chromatographic technique and has been widely applied in the field of … WebApr 18, 2024 · Moreover, an off-line hyphenated method combining high-speed counter-current chromatography, affinity-based ultrafiltration, and liquid chromatography–tandem mass spectrometry was used to identify and characterize the DCR compounds with tyrosinase inhibitory activity. Our results indicated that, DCR significantly decreased the …

WebDec 21, 2024 · Online extraction and enrichment coupling with high-speed counter-current chromatography for effective and target isolation of antitumor anthraquinones from …

WebHere, we proposed a high-speed countercurrent chromatography (HSCCC)-based method to recover extracted compounds and DES. As a proof of concept, the chrysanthemum DES … onvoy inc. - ohWebMar 1, 2024 · High-speed counter current chromatography (HSCCC) is based on the principle of liquid–liquid partitioning ( Ito & Bowman, 1971) and is well suited for … iothreadaddWebAn efficient high-speed counter-current chromatography method for the preparative separation of potential antioxidants from Paeonia lactiflora Pall. combination of in vitro … onv pcpbWebHigh-speed counter-current chromatography (HSCCC) is a liquid-liquid chromatographic technique based on solvent partition that eliminates irreversible adsorption of samples onto the solid separation materials and produces excellent sample recoveries [10]. Thus, this method has been widely used for onvoy ghostWebOct 26, 2009 · The development of HPCCC instruments combined with the limitations that chemists are experiencing with solid/liquid chromatography techniques, has created an … iot hostingWebTwo n-butyrophenone isomers with a separation factor of 1.14 were successfully separated by recycling high-speed counter-current chromatography after ten cycles. Two stibene isomers were successfully separated by preparative high-performance liquid chromatography. onv poe switchWebAll-liquid chromatography methods such as countercurrent chromatography (CCC) and centrifugal partition chromatography (CPC) have been widely used for the isolation of natural target compounds from complex plant and food samples [19,20,21,22]. CPC uses two non-miscible liquids as the stationary phase and mobile phase. iot hosts