WebSep 30, 2009 · The judicious application of intein technologies to biological problems has resulted in powerful tools for biomedical research. Inteins are intervening sequences that excise themselves from precursor proteins and ligate the surrounding sequences. ... Protein splicing occurs spontaneously, covalently attaching the target protein to the … WebSep 23, 2014 · Abstract. Alternative splicing enhances transcriptome diversity in all eukaryotes and plays a role in plant tissue identity and stress adaptation. To catalog new maize ( Zea mays) transcripts and identify genomic loci that regulate alternative splicing, we analyzed over 90 RNA-seq libraries from maize inbred lines B73 and Mo17, as well …
Small molecule strategies to harness the unfolded protein …
WebJul 24, 2024 · M Vila-Perelló, TW Muir, Biological applications of protein splicing. Cell 143, 191–200 (2010). Crossref. PubMed. Google Scholar. 32. M Muona, AS Aranko, V … WebProtein splicing is an intramolecular reaction of a particular protein in which an internal protein segment (called an intein) is removed from a precursor protein with a ligation of C-terminal and N-terminal external proteins (called exteins) on both sides.The splicing junction of the precursor protein is mainly a cysteine or a serine, which are amino acids … great new zealand toastie takeover
Protein Splicing - an overview ScienceDirect Topics
WebMar 7, 2024 · Another application developed based on expressed protein ligation and protein trans-splicing is the cyclization of polypeptide chains to increase thermodynamic and in vivo stability, which in turn can improve their biological function, making this an exciting approach for improving the efficacy of protein-based therapeutics. When using ... WebAbstract. Protein splicing domains, also called inteins, have become a powerful biotechnological tool for applications involving molecular biology and protein … WebCircular RNAs (circRNAs) are a recently discovered class of RNAs derived from protein-coding genes that have important biological and pathological roles. They are formed through backsplicing during co-transcriptional alternative splicing; however, the unified mechanism that accounts for backsplicing decisions remains unclear. Factors that … great new zealand journeys